fluorchem 8800 Search Results


fluorchem 8800 photoimager  (Alpha Innotech)
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Alpha Innotech fluorchem 8800 photoimager
Fluorchem 8800 Photoimager, supplied by Alpha Innotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fluorchem 8800 photoimager - by Bioz Stars, 2026-03
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ccd camera on a fluorchem 5500 imaging station  (Alpha Innotech)
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Alpha Innotech ccd camera on a fluorchem 5500 imaging station
Ccd Camera On A Fluorchem 5500 Imaging Station, supplied by Alpha Innotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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alpha innotech fluorchem 8800 gel box imager  (Alpha Innotech)
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Alpha Innotech alpha innotech fluorchem 8800 gel box imager
Alpha Innotech Fluorchem 8800 Gel Box Imager, supplied by Alpha Innotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fluorchem 8800 cooled-charge-coupled-device (ccd) detection system  (Alpha Innotech)
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Alpha Innotech fluorchem 8800 cooled-charge-coupled-device (ccd) detection system
Fluorchem 8800 Cooled Charge Coupled Device (Ccd) Detection System, supplied by Alpha Innotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
fluorchem 8800 cooled-charge-coupled-device (ccd) detection system - by Bioz Stars, 2026-03
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16-bit cooled ccd camera fluor chem 8000  (Alpha Innotech)
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Alpha Innotech 16-bit cooled ccd camera fluor chem 8000
16 Bit Cooled Ccd Camera Fluor Chem 8000, supplied by Alpha Innotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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16-bit cooled ccd camera fluor chem 8000 - by Bioz Stars, 2026-03
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fluorchem 8800 station  (Alpha Innotech)
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Alpha Innotech fluorchem 8800 station
Fluorchem 8800 Station, supplied by Alpha Innotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fluorchem 8800 station/product/Alpha Innotech
Average 90 stars, based on 1 article reviews
fluorchem 8800 station - by Bioz Stars, 2026-03
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densitometry fluorchem 8800  (Alpha Innotech)
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Alpha Innotech densitometry fluorchem 8800
Western blot analysis of PMCA4a expression in murine testis and epididymal tissue (A) and ELF (B, D). Twenty and 45 μg of protein/lane were loaded in A and B, respectively. PMCA4a was detected using a rabbit polyclonal anti-bovine PMCA4a antibody (1:175 dilution) in A, and a rabbit polyclonal anti-rat PMCA4a antibody (1:500 dilution) in B. A shows the presence of PMCA4a in the testis and all three epididymal regions, while B shows PMCA4a in purified ELF from each of the three epididymal regions. Blots of the latter were stripped and reprobed for β-actin as a loading control, and PMCA4a band intensities were analyzed by <t>densitometry</t> using Image J software. The relative expression in corpus/cauda compared to the caput is displayed in a bar graph in C. Values are expressed as means ± SD (n = 3), with expression highest in the cauda (*P = 0.014 versus caput). D shows immunoblotting of proteins from pellet and supernatant fractions of equivalent volumes of ELF, following ultracentrifugation. PMCA4a, seen in the pellet, is absent from the supernatant. An immunopositive band (100 kDa) which is also seen in A is of unknown origin, but most likely a breakdown product as it is much fainter in the unprocessed ELF and is not seen in B.
Densitometry Fluorchem 8800, supplied by Alpha Innotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
densitometry fluorchem 8800 - by Bioz Stars, 2026-03
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fluorchem 8800 ccd imaging system equipped with automatic bloom detection  (Alpha Innotech)
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Alpha Innotech fluorchem 8800 ccd imaging system equipped with automatic bloom detection
Western blot analysis of PMCA4a expression in murine testis and epididymal tissue (A) and ELF (B, D). Twenty and 45 μg of protein/lane were loaded in A and B, respectively. PMCA4a was detected using a rabbit polyclonal anti-bovine PMCA4a antibody (1:175 dilution) in A, and a rabbit polyclonal anti-rat PMCA4a antibody (1:500 dilution) in B. A shows the presence of PMCA4a in the testis and all three epididymal regions, while B shows PMCA4a in purified ELF from each of the three epididymal regions. Blots of the latter were stripped and reprobed for β-actin as a loading control, and PMCA4a band intensities were analyzed by <t>densitometry</t> using Image J software. The relative expression in corpus/cauda compared to the caput is displayed in a bar graph in C. Values are expressed as means ± SD (n = 3), with expression highest in the cauda (*P = 0.014 versus caput). D shows immunoblotting of proteins from pellet and supernatant fractions of equivalent volumes of ELF, following ultracentrifugation. PMCA4a, seen in the pellet, is absent from the supernatant. An immunopositive band (100 kDa) which is also seen in A is of unknown origin, but most likely a breakdown product as it is much fainter in the unprocessed ELF and is not seen in B.
Fluorchem 8800 Ccd Imaging System Equipped With Automatic Bloom Detection, supplied by Alpha Innotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
fluorchem 8800 ccd imaging system equipped with automatic bloom detection - by Bioz Stars, 2026-03
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alphaeasefc fluorchem 8800  (Alpha Innotech)
90
Alpha Innotech alphaeasefc fluorchem 8800
Western blot analysis of PMCA4a expression in murine testis and epididymal tissue (A) and ELF (B, D). Twenty and 45 μg of protein/lane were loaded in A and B, respectively. PMCA4a was detected using a rabbit polyclonal anti-bovine PMCA4a antibody (1:175 dilution) in A, and a rabbit polyclonal anti-rat PMCA4a antibody (1:500 dilution) in B. A shows the presence of PMCA4a in the testis and all three epididymal regions, while B shows PMCA4a in purified ELF from each of the three epididymal regions. Blots of the latter were stripped and reprobed for β-actin as a loading control, and PMCA4a band intensities were analyzed by <t>densitometry</t> using Image J software. The relative expression in corpus/cauda compared to the caput is displayed in a bar graph in C. Values are expressed as means ± SD (n = 3), with expression highest in the cauda (*P = 0.014 versus caput). D shows immunoblotting of proteins from pellet and supernatant fractions of equivalent volumes of ELF, following ultracentrifugation. PMCA4a, seen in the pellet, is absent from the supernatant. An immunopositive band (100 kDa) which is also seen in A is of unknown origin, but most likely a breakdown product as it is much fainter in the unprocessed ELF and is not seen in B.
Alphaeasefc Fluorchem 8800, supplied by Alpha Innotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
alphaeasefc fluorchem 8800 - by Bioz Stars, 2026-03
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alphaease fc fluorchem 8800 software, version 3.1.2  (Alpha Innotech)
90
Alpha Innotech alphaease fc fluorchem 8800 software, version 3.1.2
Western blot analysis of PMCA4a expression in murine testis and epididymal tissue (A) and ELF (B, D). Twenty and 45 μg of protein/lane were loaded in A and B, respectively. PMCA4a was detected using a rabbit polyclonal anti-bovine PMCA4a antibody (1:175 dilution) in A, and a rabbit polyclonal anti-rat PMCA4a antibody (1:500 dilution) in B. A shows the presence of PMCA4a in the testis and all three epididymal regions, while B shows PMCA4a in purified ELF from each of the three epididymal regions. Blots of the latter were stripped and reprobed for β-actin as a loading control, and PMCA4a band intensities were analyzed by <t>densitometry</t> using Image J software. The relative expression in corpus/cauda compared to the caput is displayed in a bar graph in C. Values are expressed as means ± SD (n = 3), with expression highest in the cauda (*P = 0.014 versus caput). D shows immunoblotting of proteins from pellet and supernatant fractions of equivalent volumes of ELF, following ultracentrifugation. PMCA4a, seen in the pellet, is absent from the supernatant. An immunopositive band (100 kDa) which is also seen in A is of unknown origin, but most likely a breakdown product as it is much fainter in the unprocessed ELF and is not seen in B.
Alphaease Fc Fluorchem 8800 Software, Version 3.1.2, supplied by Alpha Innotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
alphaease fc fluorchem 8800 software, version 3.1.2 - by Bioz Stars, 2026-03
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fluorchem 8800 detector  (Alpha Innotech)
90
Alpha Innotech fluorchem 8800 detector
Western blot analysis of PMCA4a expression in murine testis and epididymal tissue (A) and ELF (B, D). Twenty and 45 μg of protein/lane were loaded in A and B, respectively. PMCA4a was detected using a rabbit polyclonal anti-bovine PMCA4a antibody (1:175 dilution) in A, and a rabbit polyclonal anti-rat PMCA4a antibody (1:500 dilution) in B. A shows the presence of PMCA4a in the testis and all three epididymal regions, while B shows PMCA4a in purified ELF from each of the three epididymal regions. Blots of the latter were stripped and reprobed for β-actin as a loading control, and PMCA4a band intensities were analyzed by <t>densitometry</t> using Image J software. The relative expression in corpus/cauda compared to the caput is displayed in a bar graph in C. Values are expressed as means ± SD (n = 3), with expression highest in the cauda (*P = 0.014 versus caput). D shows immunoblotting of proteins from pellet and supernatant fractions of equivalent volumes of ELF, following ultracentrifugation. PMCA4a, seen in the pellet, is absent from the supernatant. An immunopositive band (100 kDa) which is also seen in A is of unknown origin, but most likely a breakdown product as it is much fainter in the unprocessed ELF and is not seen in B.
Fluorchem 8800 Detector, supplied by Alpha Innotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
fluorchem 8800 detector - by Bioz Stars, 2026-03
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alphaeascfc fluorchem 8800 imaging system  (Alpha Innotech)
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Alpha Innotech alphaeascfc fluorchem 8800 imaging system
Western blot analysis of PMCA4a expression in murine testis and epididymal tissue (A) and ELF (B, D). Twenty and 45 μg of protein/lane were loaded in A and B, respectively. PMCA4a was detected using a rabbit polyclonal anti-bovine PMCA4a antibody (1:175 dilution) in A, and a rabbit polyclonal anti-rat PMCA4a antibody (1:500 dilution) in B. A shows the presence of PMCA4a in the testis and all three epididymal regions, while B shows PMCA4a in purified ELF from each of the three epididymal regions. Blots of the latter were stripped and reprobed for β-actin as a loading control, and PMCA4a band intensities were analyzed by <t>densitometry</t> using Image J software. The relative expression in corpus/cauda compared to the caput is displayed in a bar graph in C. Values are expressed as means ± SD (n = 3), with expression highest in the cauda (*P = 0.014 versus caput). D shows immunoblotting of proteins from pellet and supernatant fractions of equivalent volumes of ELF, following ultracentrifugation. PMCA4a, seen in the pellet, is absent from the supernatant. An immunopositive band (100 kDa) which is also seen in A is of unknown origin, but most likely a breakdown product as it is much fainter in the unprocessed ELF and is not seen in B.
Alphaeascfc Fluorchem 8800 Imaging System, supplied by Alpha Innotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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alphaeascfc fluorchem 8800 imaging system - by Bioz Stars, 2026-03
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Image Search Results


Western blot analysis of PMCA4a expression in murine testis and epididymal tissue (A) and ELF (B, D). Twenty and 45 μg of protein/lane were loaded in A and B, respectively. PMCA4a was detected using a rabbit polyclonal anti-bovine PMCA4a antibody (1:175 dilution) in A, and a rabbit polyclonal anti-rat PMCA4a antibody (1:500 dilution) in B. A shows the presence of PMCA4a in the testis and all three epididymal regions, while B shows PMCA4a in purified ELF from each of the three epididymal regions. Blots of the latter were stripped and reprobed for β-actin as a loading control, and PMCA4a band intensities were analyzed by densitometry using Image J software. The relative expression in corpus/cauda compared to the caput is displayed in a bar graph in C. Values are expressed as means ± SD (n = 3), with expression highest in the cauda (*P = 0.014 versus caput). D shows immunoblotting of proteins from pellet and supernatant fractions of equivalent volumes of ELF, following ultracentrifugation. PMCA4a, seen in the pellet, is absent from the supernatant. An immunopositive band (100 kDa) which is also seen in A is of unknown origin, but most likely a breakdown product as it is much fainter in the unprocessed ELF and is not seen in B.

Journal: Biology of Reproduction

Article Title: Plasma Membrane Ca 2+ -ATPase 4 in Murine Epididymis: Secretion of Splice Variants in the Luminal Fluid and a Role in Sperm Maturation 1

doi: 10.1095/biolreprod.113.108712

Figure Lengend Snippet: Western blot analysis of PMCA4a expression in murine testis and epididymal tissue (A) and ELF (B, D). Twenty and 45 μg of protein/lane were loaded in A and B, respectively. PMCA4a was detected using a rabbit polyclonal anti-bovine PMCA4a antibody (1:175 dilution) in A, and a rabbit polyclonal anti-rat PMCA4a antibody (1:500 dilution) in B. A shows the presence of PMCA4a in the testis and all three epididymal regions, while B shows PMCA4a in purified ELF from each of the three epididymal regions. Blots of the latter were stripped and reprobed for β-actin as a loading control, and PMCA4a band intensities were analyzed by densitometry using Image J software. The relative expression in corpus/cauda compared to the caput is displayed in a bar graph in C. Values are expressed as means ± SD (n = 3), with expression highest in the cauda (*P = 0.014 versus caput). D shows immunoblotting of proteins from pellet and supernatant fractions of equivalent volumes of ELF, following ultracentrifugation. PMCA4a, seen in the pellet, is absent from the supernatant. An immunopositive band (100 kDa) which is also seen in A is of unknown origin, but most likely a breakdown product as it is much fainter in the unprocessed ELF and is not seen in B.

Article Snippet: Protein bands were analyzed by densitometry (FluorChem 8800; Alpha Innotech Corp.), and the ratio of PMCA4a to the loading controls was determined and plotted.

Techniques: Western Blot, Expressing, Purification, Control, Software